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SETSCI - Volume 1 (2017)
ISMSIT2017 - International Symposium on Multidisciplinary Studies and Innovative Technologies, Tokat, Turkey, Dec 02, 2017

Chromatographic Performance of Weak Cation Exchanger Carrying Molecular Brushes Synthesized by Atom Transfer Radical Polymerization
Berna S. Kaya1*, Ali Tuncel2
1Cumhuriyet University, Sivas, Turkey
2Hacettepe University, Ankara, Turkey
* Corresponding author: bernasaracoglu@hotmail.com
Published Date: 2017-12-08   |   Page (s): 310-313   |    647     7
ABSTRACT The column materials in the form of monodisperse and porous particles were synthesized with regard to test their column performances in Ion Exchange Chromatography (IEC) mode. Particles in the form of poly(glycidil methacrylate-coethyleneglycol dimethacrylate) poly(GMA-co-EDM) copolymer used for starting materials in the synthesis, were maintained by “multi-stage microsuspension polymerization”. Particle characterization was assessed by Scanning Electron Microscopy (SEM), BET surface area and porosity measurement system and FTIR spectroscopy. Weak cation-exchange carboxyl (- COOH) group on monodisperse and porous poly(DHPM-co-EDM) particles, used for ion-exchange chromatography, was
synthesized by “Atom Transfer Radical Polymerization” (ATRP) method. The investigation of column performance concluded that weak cation-exchanged columns, synthesized by ATRP (150-300 µm) showed low and stable theoretical plate heights and high peak resolution values compared to literature knowledge. Protein recovery data in ion-exchange column, which is synthesized by ATRP (94-100%) polymerization technique were determined as similar as quantitative values for all proteins. “Nano-scale change” of ligand length determined the chromatographic performance of functional based ion-exchanged stable phase. Chromatographic studies demonstrated that the length of polymeric ligand, which can be controlled by ATRP polymerization, affected decrease of retention time without any change of column reverse pressure values, chromatographic separation ability (peak resolution and plate height) and analyte recovery from column.  
KEYWORDS Atom Transfer Radical Polymerization (ATRP), High Performance Liquid Chromatograpy (HPLC), Ion Exchange Chromatography (IEC), Monodisperse and porous polymeric particles
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