Journals Books 2687-5527 doi.org/10.36287/setsci
Latest Issue Archive Future Issues About Us
Conference Proceedings

SETSCI - Volume 1 (2017)
ISMSIT2017 - International Symposium on Multidisciplinary Studies and Innovative Technologies, Tokat, Turkey, Dec 02, 2017

Chromatographic Performance of Weak Cation Exchanger Carrying Molecular Brushes Synthesized by Atom Transfer Radical Polymerization
Berna S. Kaya1*, Ali Tuncel2
1Cumhuriyet University, Sivas, Turkey
2Hacettepe University, Ankara, Turkey
* Corresponding author: bernasaracoglu@hotmail.com
Published Date: 2017-12-08   |   Page (s): 310-313   |    511     6
ABSTRACT The column materials in the form of monodisperse and porous particles were synthesized with regard to test their column performances in Ion Exchange Chromatography (IEC) mode. Particles in the form of poly(glycidil methacrylate-coethyleneglycol dimethacrylate) poly(GMA-co-EDM) copolymer used for starting materials in the synthesis, were maintained by “multi-stage microsuspension polymerization”. Particle characterization was assessed by Scanning Electron Microscopy (SEM), BET surface area and porosity measurement system and FTIR spectroscopy. Weak cation-exchange carboxyl (- COOH) group on monodisperse and porous poly(DHPM-co-EDM) particles, used for ion-exchange chromatography, was
synthesized by “Atom Transfer Radical Polymerization” (ATRP) method. The investigation of column performance concluded that weak cation-exchanged columns, synthesized by ATRP (150-300 µm) showed low and stable theoretical plate heights and high peak resolution values compared to literature knowledge. Protein recovery data in ion-exchange column, which is synthesized by ATRP (94-100%) polymerization technique were determined as similar as quantitative values for all proteins. “Nano-scale change” of ligand length determined the chromatographic performance of functional based ion-exchanged stable phase. Chromatographic studies demonstrated that the length of polymeric ligand, which can be controlled by ATRP polymerization, affected decrease of retention time without any change of column reverse pressure values, chromatographic separation ability (peak resolution and plate height) and analyte recovery from column.  
KEYWORDS Atom Transfer Radical Polymerization (ATRP), High Performance Liquid Chromatograpy (HPLC), Ion Exchange Chromatography (IEC), Monodisperse and porous polymeric particles
REFERENCES [1] Ellingsten T., Aune O., Ugelstad J., Hagen S., “Monosized Stationary Phases For Chromatography”, Journal of Chromatography, 535, 147- 161, 1990.

[2] Wang j., Matyjaszewski K., “Living"/Controlled Radical Polymerization. Transition-Metal-Catalyzed Atom Transfer Radical Polymerization in the Presence of a Conventional Radical Initiator”, Macromolecules, 28, 7572-7573, 1995.

[3] Galia M., Svec F., Frechet J. M. J., “Monodisperse Polymer Beads As Packing Material For High Performance Chromatography: Effect of Divinylbenzene Content on the Porous and Chromotagraphic Properties of Poly(styrene-co-divinylbenzene) Beads Prepared in the Presence of Lineer Polystyrene as a Porogen”, Journal of Polymer Science: Part A: Polymer Chemistry, 32, 2169-2175, 1994.

[4] Svec F., Frechet J. M. J., “New Designs of Macroporous Polymers and Supports: From Seperation to Biocatalysis”, Science, 273, 205-211, 1996.

[5] Hossoya K., Teramachi M., Tanaka N., Kobayashi A., Kanda T., Ohtsu Y., “Preparation Strategy for Uniformly Sized, Polymer-Beads HPLC Packing Materials Having Practically Acceptable Column Efficiency. 1. Copolymerization Technique”, Anal. Chem., 73, 5852, 2001.

[6] Smigol V., Svec F., Frechet J.M.J., “2-Dimensional HighPerformance Liquid-Chromatography Using Monodisperse Polymer Beads Containing Segregated Chemistries Prepared By Pore-Size Specific Functionalization - Single-Column Combinations Of Size Exclusion Or İon-Exchange With Reversed-Phase Chromatography”, Analytical Chemistry 66 (23), 4308-4315, 1994.

[7] Smigol V.; Svec, F., Frechet, J.M.J., “High-Performance Liquid Chromatography of Complex mixtures Using Monodisperse DualChemistry Polymer Beads Prepared by a Pore-Size-Specific Functionalization Process. A Single Column Combination of Hydrophobic Interaction and Reversed-Phase Chromatography”,
Analytical Chemistry, 66, 2129, 1994.

[8] Lewandowski K.; Svec F., Frechet J.M.J., “Polar, A Novel Polar Seperation Medium For The Size Exclusion Chromatography of Small Molecules: Uniformly Sized, Porous Poly(vinylphenol-codivinylbenzene) Beads”, J. Liq. Chrom. & Rel. Technol., 20, 227, 1997.

[9] Lewandowski K.; Svec F., Frechet J.M.J., “Polar, Monodisperse, Reactive Beads from Functionalized Metacrylate Monomers by staged Templated Suspension Polymerization”, Chem. Mater., 10, 385, 1998.

[10] Unsal E., Elmas B., Çağlayan B., Tuncel M., Patır S., Tuncel A., “Preparation of An Ion Exchange Chromatographic Support By A New “Grafting From” Strategy Based on Atom Transfer Radical Polymerization”, Analytical Chemistry, 78, 1-8, 2006.


SET Technology - Turkey
eISSN  : 2687-5527    
DOI : doi.org/10.36287/setsci

E-mail : info@set-science.com
+90 533 2245325

Tokat Technology Development Zone Gaziosmanpaşa University Taşlıçiftlik Campus, 60240 TOKAT-TURKEY
©2018 SET Technology