Journals Books 2687-5527 doi.org/10.36287/setsci
Latest Issue Archive Future Issues About Us
Conference Proceedings

SETSCI - Volume 4 (6) (2019)
ISAS WINTER-2019 (ENS) - 4th International Symposium on Innovative Approaches in Engineering and Natural Sciences, Samsun, Turkey, Nov 22, 2019

Phylogenetic Analysis of Streptomyces sp. H2AK isolated from soil in Kuşadası
Demet Tatar1, Aysel Veyisoğlu2*
1Hitit University, Çorum, Turkey
2Sinop University, Sinop, Turkey
* Corresponding author: aveyisoglu@sinop.edu.tr
Published Date: 2019-12-22   |   Page (s): 420-422   |    187     6
https://doi.org/10.36287/setsci.4.6.108

ABSTRACT Streptomyces is a genus of Gram-positive bacteria that has a filamentous form similar to fungi. Streptomyces produces bioactive secondary metabolites, such as antifungals, antivirals, antitumorals and various antibiotics (Procópio et al., 2012). The aim of this study is to carry out phylogenetic analysis of Streptomyces sp. H2AK isolated from soil sample taken from the Kuşadası located near Aydın province. Streptomyces sp. H2AK, was picked after 14 days of incubation at 28˚C on Humic acid-vitamin agar containing Cycloheximide (50 µg/ml) ve Nalidixic acid (10 μg/ml). Genomic DNA isolation was performed according to the method of Pitcher et al. (1989). The 16S rRNA gene was amplified by PCR using universal primers 27f and 1525r. Phylogenetic analyses were performed by using three different algorithms with MEGA 7 software. H2AK showed the highest 16S rRNA gene sequence similarity with Streptomyces marokkonensis Ap1T (98.90 %). When the polyphasic taxonomic analyses were completed, Streptomyces sp. H2AK isolate may be introduced into the literature as a new species of the genus Streptomyces. This study was supported by Hitit University (ODMYO19003.18.001). 
KEYWORDS Taxonomy, Phylogenetic Analysis, 16S rRNA Gene, Streptomyces
REFERENCES Pitcher D. G., Saunders N. A., and Owen R. J., Rapid extraction of bacterial genomic DNA with guanidium thiocyanate, Lett. Appl. Microbiol, 8, 151-156, 1989.
[2] URL-1: https://aydin.ktb.gov.tr/TR-64364/kusadasi.html (Available: 30 Nov 2019).
[3] Stackebrandt, E., Rainey, F. A. & Ward-Rainey, N. L., Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47, 479–491, 1997.
[4] de Lima Procópio, R. E., Da Silva, I. R., Martins, M. K., De Azevedo, J. L., and De Araújo, J. M., Antibiotics produced by Streptomyces. Braz. J. Infect. Dis. 16, 466–471. doi: 10.1016/j.bjid.2012.08.014, 2012.
[5] Chater, K.F, Recent advances in understanding Streptomyces [version 1; referees: 4 approved] F1000Research, 5(F1000 Faculty Rev):2795 (doi: 10.12688/f1000research.9534.1), 2016.
[6] URL-2:http://www.bacterio.net/streptomyces.html (Available: 30 Nov 2019).
[7] Hayakawa, M., Nonomura, H, Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. J Ferment Technol, 65, pp.501-509, 1987.
[8] Yoon SH, Ha SM, Kwon S, Lim J, Kim Y, Seo H, Chun J, Introducing EzBioCloud: A taxonomically united database of 16S rRNA and whole genome assemblies. Int J Syst Evol Microbiol 67:1613-1617, 2017.
[9] Kumar S, Stecher G, Tamura K,. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33:1870-1874, 2016.
[10]Saitou N, Nei M,. The neighbor-joining method. A new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425, 1987.
[11]Felsenstein J,. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17: 368–376, 1981.
[12]Kluge AG, Farris FS,. Quantitative phyletics and the evolution of anurans, Syst Zool 18: 1–32, 1969.
[13]Jukes TH, Cantor CR,. Evolution of protein molecules. In Mammalian Protein Metabolism vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press, 1969.
[14]Felsenstein J,. Confidence limits on phylogeny: an approach using the bootstrap. Evolution 39: 783–791, 1985.
[15] Lane DJ,. 16S/23S rRNA sequencing. In: Nucleic acid techniques in bacterial systematics. Stackebrandt, E., and Goodfellow, M., eds., John Wiley and Sons, New York, NY, pp. 115-175, 1991.
[16]Buchholz-Cleven BEE, Rattunde B & Straub KL,. Screening for genetic diversity of isolates of anaerobic Fe(II)-oxidizing bacteria using DGGE and whole-cell hybridization. Syst. Appl. Microbiol. 20: 301-309, 1997.
[17]Chun J, Goodfellow M,. A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45: 240–245, 1995


SET Technology - Turkey

eISSN  : 2687-5527    
DOI : doi.org/10.36287/setsci

E-mail : info@set-science.com
+90 533 2245325

Tokat Technology Development Zone Gaziosmanpaşa University Taşlıçiftlik Campus, 60240 TOKAT-TURKEY
©2018 SET Technology