Open Access
Protective Effect of Melatonin on Bone Tissue in Elderly Diabetic Female Rats
Ömer Ünal1, Saltuk Buğra Baltacı2, Nilüfer  Akgün Ünal3, Rasim Mogulkoc4, Abdülkerim Kasım Baltacı5*
1Selcuk University, Konya, Turkey
2Selcuk University, Konya, Turkey
3Selcuk University, Konya, Turkey
4Selcuk University, Konya, Turkey
5Selcuk University, Konya, Turkey
* Corresponding author:

Presented at the 4th International Symposium on Innovative Approaches in Health and Sports Sciences (ISAS WINTER-2019 (HSS)), Samsun, Turkey, Nov 22, 2019

SETSCI Conference Proceedings, 2019, 12, Page (s): 22-23 ,

Published Date: 23 December 2019    | 834     39


The aim of this study was to investigate the effects of melatonin administraton on bone tissue damage  in a diabetic elderly female rat model. The study was performed on female aged rats (16 months old) who were provided by The Experimental Medicine Research and Application Center of Selçuk University.  A total of 24 elderly female rats were divided into 4 groups: Group 1. Control, Group 2. Control + Melatonin, Group 3. Diabetes, Group 4. Diabetes + Melatonin. At the end of the study,  MDA ve GSH levels were determined by ELISA, on bone tissue samples obtained from the  animals  sacrificed  under general anesthesia. In our study, the highest bone MDA values were found in the Diabetes Group. MDA values of the Diabetes + Melatonin  group were significantly lower than all the other groups. In our study, the highest bone GSH values were found in the Diabetes + Melatonin group and the lowest bone GSH levels were found in the Diabetes Group. The findings of our study show that oxidative stress in bone tissue can be prevented by melatonin supplementation in diabetic elderly female rats.

Keywords - elderly female rats, diabetes mellitus, melatonin, bone tissue, oxidative stress


[1] C. Zhao, G. Liu, Y. Zhang, G. Xu, X. Yi, J. Liang, Y. Yang, J. Liang, C. Ma, Y. Ye, M. Yu, X. Qu. “Association between serum levels of bone turnover markers and bone mineral density in men and women with type 2 diabetes mellitus.” J Clin Lab Anal e23112 2019.
[2] CE. Murray, CM. Coleman. “Impact of Diabetes Mellitus on Bone Health.” Int J Mol Sci; 20(19). pii: E4873 2019.
[3] M. Bicer, SB. Baltaci, S. Patlar, R. Mogulkoc, AK. Baltaci. “Melatonin has a protective effect against lipid peroxidation in the bone tissue of diabetic rats subjected to acute swimming exercise.” Horm Mol Biol Clin Investig;34(2). pii: /j/hmbci.2018.34.issue-2 2018.
[4] K. Baba, AJ. Davidson, G. Tosini. “Melatonin entrains PER2:LUC bioluminescence circadian rhythm in the mouse cornea.” Invest Ophthalmol Vis Sci;56:4753-4758 2015.
[5] B.Claustrat, J. Leston. “Melatonin: Physiological effects in humans.” Neurochirurgie;61:77-84 2015.
[6] H. Gurer-Orhan, S. Suzen. “Melatonin, its metabolites and its synthetic analogs as multi-faceted compounds: antioxidant,prooxidant and inhibitor of bioactivation reactions.” Curr Med Chem;22:490-499 2015.
[7] E. Tan, EM. Scott. “Circadian rhythms, insulin action, and glucose homeostasis.” Curr Opin Clin Nutr Metab Care;17:343-348 2014.
[8] BL. Furman. “Streptozotocin-Induced Diabetic Models in Mice and Rats”. Curr Protoc Pharmacol;70:5.47.1-20 2015.

Copyright © 2024 SETECH
Tokat Technology Development Zone Gaziosmanpaşa University Taşlıçiftlik Campus, 60240 TOKAT-TÜRKİYE