Journals Books 2687-5527
Latest Issue Archive Future Issues About Us
Conference Proceedings

SETSCI - Volume 4 (9) (2019)
ISAS WINTER-2019 (HSS) - 4th International Symposium on Innovative Approaches in Health and Sports Sciences, Samsun, Turkey, Nov 22, 2019

The Effects of Resveratrol Supplementation on Bone Tissue SIRT1 levels in old Female Rats with Diabetic
Kübra Kıyar1, Ömer Ünal2, Saltuk Buğra Baltacı3, Nilüfer Akgün Ünal4, Rasim Mogulkoc5, Abdülkerim Kasım Baltacı6*
1Selcuk University, Konya, Turkey
2Selcuk University, Konya, Turkey
3Selcuk University, Konya, Turkey
4Selcuk University, Konya, Turkey
5Selcuk University, Konya, Turkey
6Selcuk University, Konya, Turkey
* Corresponding author:
Published Date: 2019-12-23   |   Page (s): 24-25   |    426     28

ABSTRACT The aim of this study was to investigate how resveratrol administration  diabetic elderly female  rats affects the levels of bone tissue SIRT1. The study was performed on female aged rats (16 months old) who were provided by The Experimental Medicine Research and Application Center of Selçuk University.  A total of 24 elderly female rats were divided into 4 groups: Group 1. Control, Group 2. Control + Resveratrol, Group 3. Diabetes, Group 4. Diabetes + Resveratrol. In order to induce diabetes, the animals were intraperitoneally (IP) injected with 40 mg/kg streptozotocin (STZ). The animals were supplemented with 5 mg/kg/day resveratrol IP for 4 weeks.  At the end of the study, SIRT1 protein gene expression  were determined by PCR, on bone tissue samples obtained from the  animals  sacrificed  under general anesthesia. In our study, the highest bone tissue SIRT1 expression values were obtained in the diabetes + resveratrol group (G4) and the lowest bone SIRT 1 levels were found in the diabetes group (G3). Our results suggest that resveratrol supplementation increases bone SIRT1 expression in diabetic elderly female rats
KEYWORDS old female rats, diabetes mellitus, resveratrol supplementation, SIRT1, bone tissue
REFERENCES [1] O. Tell-Lebanon, P. Rotman-Pikielny. “Osteoporosis and diabetes - in which way are they related?.” Harefuah ;155(11):697-701 2016.
[2] G. Giovos, MP. Yavropoulou, JG. Yovos. “The role of cellular senescence in diabetes mellitus and osteoporosis: molecular pathways and potential interventions.” Hormones (Athens) Nov 8. doi: 10.1007/s42000-019-00132-6, 2019.
[3] Y. Fei, E. Shimizu, MW. McBurney, NC. Partridge. “Sirtuin 1 is a negative regulator of parathyroid hormone stimulation of matrix metalloproteinase 13 expression in osteoblastic cells: role of sirtuin 1 in the action of PTH on osteoblasts.” J Biol Chem ;290(13):8373-8382 2015.
[4] QB. Zhang, W. Cao, YR. Liu, SM. YY. Cui, Yan. “Effects of Sirtuin 1 on the proliferation and osteoblastic differentiation of periodontal ligament stem cells and stem cells from apical papilla.” Genet Mol Res ;15(1). doi: 10.4238/gmr.15015234 2016.
[5] Y. Huang, J. Huo, FQ. Liu, J. Liu, XJ. Zhang, CH. Guo, LH. Song. “Resveratrol Promotes in vitro Differentiation of Osteoblastic MC3T3-E1 Cells via Potentiation of the Calcineurin/NFATc1 Signaling Pathway.” Biochemistry (Mosc) ;84(6):686-692 2019.
[6] M. Zhao, SY. Ko, IR. Garrett, GR. Mundy, GE. Gutierrez, JR. Edwards. “The polyphenol resveratrol promotes skeletal growth in mice through a sirtuin 1-bone morphogenic protein 2 longevity axis.” Br J Pharmacol ;175(21):4183-4192 2018.
[7] BL. Furman. “Streptozotocin-Induced Diabetic Models in Mice and Rats”. Curr Protoc Pharmacol;70:5.47.1-20 2015.

SET Technology - Turkey

eISSN  : 2687-5527    

E-mail :
+90 533 2245325

Tokat Technology Development Zone Gaziosmanpaşa University Taşlıçiftlik Campus, 60240 TOKAT-TURKEY
©2018 SET Technology