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SETSCI - Volume 4 (9) (2019)
ISAS WINTER-2019 (HSS) - 4th International Symposium on Innovative Approaches in Health and Sports Sciences, Samsun, Turkey, Nov 22, 2019

Pseudomonas aeruginosa detection methods from ground beef and chicken meat samples
Belgin Sırıken1, Gökhan İnat2, Ceren Başkan3*
1Ondokuz Mayıs University, Samsun, Turkey
2Ondokuz Mayıs University, Samsun, Turkey
3Amasya University, Amasya, Turkey
* Corresponding author: bsiriken@yahoo.com
Published Date: 2019-12-23   |   Page (s): 136-140   |    226     6
https://doi.org/10.36287/setsci.4.9.082

ABSTRACT In this study, chicken and ground beef samples purchased from supermarkets and butchers in Samsun Province were analyzed for presence of Pseudomonas (P.) aeruginosa. In this case, classical culture technique were applied with molecular techniques. Isolation was performed on Pseudomonas CN Selective Agar [Oxoid SR 102E, suppl. Pseudomonas Agar base-(Oxoid CM 0559)] (EN ISO 13720). The plates was incubated aerobically for 24-48 h at 37 °C. After the incubation, up to five susceptible colonies grown on the Pseudomonas CN Selective Agar were subcultured onto Tryptone Soya Agar plates (TSA-Oxoid-CM0131-L21). The presumptive P. aeruginosa colonies were tested with the Gram staining, oxidase (Oxoid BR 64) and catalase test. In addition, the colonies were streaked onto Endo Agar Base (Oxoid, CM0479, suppl. BR0050). For confirmation of the isolates for being P. aeruginosa at molecular levels, two types of genes, namely the oprL and PA-SS (16 S rDNA) gene region, were performed by a single-targeted PCR assay. As a result, 140 isolates were obtained and identified as P. aeruginosa using classic culture technique, and 50 out of 140 isolates were identified P. aeruginosa isolates using PCR technique. Between two genes regions, although only oprL gene detected in the 50 isolates, PA-SS (16 S rDNA) gene was not detected in any of the isolates.
KEYWORDS Pseudomonas aeruginosa, ground beef, chicken meat, oprL gene, PA-SS (16 S rDNA) gene
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