Presented at the 2nd International Symposium on Innovative Approaches in Scientific Studies (ISAS2018-Winter), Samsun, Turkey, Nov 30, 2018

SETSCI Conference Proceedings, 2018, 3, Page (s): 929-932 , https://doi.org/

Published Date: 31 December 2018    | 441     9

Chicken and ground beef samples collected from Samsun province of Turkey were analyzed for P. aeruginosa. The
isolates were obtained from classic culture technique using Pseudomonas Agar base (Oxoid CM 0559, suppl. SR 102E). After
isolation, Gram staining, oxidase and catalase test and Endo Agar Base were used for the confirmation of the isolates. Catalase
and oxidase positive, Gram negative and colorless colonies (up to 5) on the Endo Agar base were chosen as a presumptive P.
aeruginosa. Then, for the confirmation of the isolates based on molecular level, oprL gene (species-specific) was detected in the
isolates using PCR assay. The oprL gene present isolates were evaluated as P. aeroginosa. In this way, 23 P.aeruginosa isolates
were obtained. Then, antibiotic resistance properties of the isolates were investigated using disk diffusion test. For this, a total 8
antibiotics- ceftazidime (30 µg), colistin (10 µg), fluoroquinolones (levofloxacin) (5 µg), tobramycin (10 µg), gentamicin (10
µg), meropenem (10 µg), imipenem (10 µg) and piperacillin-tazobactam (100/10 µg)- were used as antibiotics. For determination
of antibiotic resistance properties of the isolates, Mueller Hinton Agar was used. According to analyzed results; 5 out of 23
isolates (21.73%) were resistance to one or two antibiotics. One isolate was resistance against gentamycin, 4 (17.39%) isolates
resistance to colistin. On the other hand, one of them was resistance to both gentamycin and tobramycin, too. So, 1 of 23 (4.34%)
isolate resistance to aminoglycoside group antibiotics (gentamicin and tobramycin). However, 18 isolates were sensitive to all
tested antibiotics. No multi antibiotic resistance isolates were present  

Keywords - Chicken meat, ground beef, Pseudomonas aeruginosa, oprL gene, antibiotic resistance

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