Presented at the 4th International Symposium on Innovative Approaches in Engineering and Natural Sciences (ISAS WINTER-2019 (ENS)), Samsun, Turkey, Nov 22, 2019

SETSCI Conference Proceedings, 2019, 9, Page (s): 485-489 , https://doi.org/10.36287/setsci.4.6.135

Published Date: 22 December 2019    | 845     11

In the present study, the volatile compounds of essential oil of Lavandula stoechas L. (Lavender) was analysed by gas chromatography-mass spectrometry (GC-MS) using the Nist and Willey libraries. It was determined that the main components of L. stoechas were linalyl acetate (36.31%) and linalool (34.18%). Inhibition and antimicrobial activity of volatile oil of the plant against Escherichia coli (ATCC 25293), Staphylococcus aureus (ATCC25925) and Candida albicans were examined by microbroth dilution method. L. stoechas EO (Essential Oil) exhibited antimicrobial activity with MIC of between 14.8 and 32.5 µg/mL. The lowest antimicrobial activity were determined against E. coli in L. stoechas EO (MIC= 32.5 µg/mL), while maximum MIC were noticed as 14.8 µg/mL against C. albicans. The most cell inhibition were noted as 91.76% in C. albicans at the concentration of 4500 µg/mL of L. stoechas. The reductions in living cell number were obtain as 0.54 log in E. coli, 1.04 log in S. aureus and 1.08 log in C. albicans at the same concentration of 4.5 µg/mL. This study showed the strong antimicrobial performance of L. stoechas EO in detail. This study showed that L. stoechas EO inhibits the growth of microorganisms depending on concentration.  

Keywords - Lavandula stoechas, antimicrobial action, cell inhibition, reduction of living cell

[1] A. Lopez, J.B. Hudson, G.H.N. Towers, “Antiviral and antimicrobial activities of Colombian medicinal plants”. J Ethnopharmacol., vol. 77, pp. 189-96, Oct. 2001.
[2] N.Y.O. Muyima, G. Zulu, T. Bhengu, D. Popplewell, “The potential application of some novel essential oils as natural cosmetic preservatives in an aqueous cream formulation,” Flavour Fragr. J., vol. 17, pp. 258-266, March. 2002.
[3] S. De Rapper, G. Kamatou, A. Viljoen, S. Van Vuuren, “The in vitro antimicrobial activity of Lavandula angustifolia essential oil in combination with other aroma-therapeutic oils,” Evid-Based Compl Altern Med., Vol. 2013:852049, Apr. 2013.
[4] C. Jianu, G. Pop, A.T. Gruia, F.G. Horhat, “Chemical composition and antimicrobial activity of essential oils of lavender (Lavandula angustifolia) and lavandin (Lavandula x intermedia) grown in Western Romania,” Int J Agric Biol, Vol. 15, pp. 772-776, March. 2013.
[5] V. Hajhashemi, A. Ghannadi, B. Sharif, “Anti-inflammatory and analgesic properties of the leaf extracts and essential oil of Lavandula angustifolia Mill,”. J Ethnopharmacol., vol. 89, pp. 67-71. Nov. 2003.
[6] I. Spiridon, S. Colceru, N. Anghel, C.A. Teaca, R. Bodirlau, A. Armatu, “Antioxidant capacity and total phenolic contents of oregano (Origanum vulgare), lavender (Lavandula angustifolia) and lemon balm (Melissa officinalis) from Romania,” Nat Prod Res. Vol. 25, pp. 1657-61, Jun. 2011.
[7] M. Zuzarte, M.J. Gonçalves, C. Cavaleiro, J. Canhoto, L. Vale-Silva, M.J. Silva, E. Pinto, L. Salgueiro, “Chemical composition and antifungal activity of the essential oils of Lavandula viridis l’Her,”. J Med Microbiol., Vol. 60, pp. 612-618, May. 2011.
[8] M. Zuzarte, L. Vale-Silva, M.J. Gonçalves, C. Cavaleiro, S. Vaz, J. Canhoto, E. Pinto, L. Salgueiro, “Antifungal activity of phenolic-rich Lavandula multifida L. Essential oil,” Eur J Clin Microbiol Infect Dis., Vol. 31, pp. 1359-66, Oct. 2012.
[9] K. Canli, A. Yetgin, A. Benek, M.E. Bozyel, E.M. Altuner, "In Vitro Antimicrobial Activity Screening of Ethanol Extract of Lavandula stoechas and Investigation of Its Biochemical Composition," Adv Pharmacol Sci, Volume 2019, ID 3201458, janu. 2019.
[10] A.H. Gilani, N. Aziz, M.A. Khan, F. Shaheen, Q. Jabeen, B.S. Siddiqui, J.W. Herzig, “Ethnopharmacological evaluation of the anticonvulsant, sedative and antispasmodic activities of Lavandula stoechas L,” J Ethnopharmacol., vol 71, pp. 161-167, Nov. 2000.
[11] Dalynn Biologicals, McFarland Standard, Cat no: TM50-TM60, 2014.
[12] T. Patton, J. Barett, J. Brennan, N. Moran, “Use of a spectrophotometric bioassay for determination of microbial sensitivity to manuka honey,” J Microbiol Methods., Vol. 64, pp. 6484-6495, Apr. 2006.
[13] Y. Sıcak, E.A. Erdogan Eliuz, “Determination of the phytochemical profile, in vitro the antioxidant and antimicrobial activities of essential oil from Arbutus andrachne L. wood growing in Turkey,” Turkish J Foresty., Vol. 20, pp. 57-61, March. 2019.
[14] A.T. Peana, P.S. D’Aquila, F. Panin, G. Serra, P. Pippia, M.D.L. Moretti, “Anti-inflammatory activity of linalool and linalyl acetate constituents of essential oils,”. Phytomedicine., Vol., 9, pp. 721-726. 2002.
[15] A.C. Gören, G. Topçu, G. Bilsel, M. Bilsel, Z. Aydoǧmuş, J.M. Pezzuto, “The chemical constituents and biological activity of essential oil of Lavandula stoechas ssp. stoechas,” Z Naturforschung C., Vol., 57, pp. 9-10, June. 2002.
[16] A. Angioni, A. Barra, V. Coroneo, S. Dessi, P. Cabras, “Chemical composition, seasonal variability, and antifungal activity of Lavandula stoechas L. ssp. stoechas essential oils from stem/leaves and flowers,” J Agric Food Chem., Vol. 54, pp. 4364-70, May. 2006.
[17] N. Bouzouita, F. Kachouri, M. Hamdi, M.M. Chaabouni, R. Ben Aissa, S. Zgoulli, “Volatile constituents and antimicrobial activity of Lavandula stoechas L. oil from Tunisia,” J Essent Oil Res., Vol. 17, pp. 584-586., Nov. 2005.
[18] E.M. Soylu, S. Soylu, S. Kurt, “Antimicrobial activities of the essential oils of various plants against tomato late blight disease agent Phytophthora infestans,” Mycopathologia., Vol. 161, pp. 119-128, Oct. 2006.
[19] T. Benabdelkader, A. Zitouni, Y. Guitton, F. Jullien, D. Maitre, H. Casabianca, L. Legendre, A. Kameli, “Essential oils from wild populations of algerian Lavandula stoechas L. Composition, chemical variability, and in vitro biological properties,” Chem Biodivers., Vol. 8, pp. 937-953, May, 2011
[20] I. Dadalioǧlu, G.A. Evrendilek, “Chemical compositions and antibacterial effects of essential oils of Turkish oregano (Origanum minutiflorum), bay laurel (Laurus nobilis), Spanish lavender (Lavandula stoechas L.), and fennel (Foeniculum vulgare) on common foodborne pathogens,” J Agric Food Chem., Vol., 52(26): 8255-60, Nov. 2004.
[21] M. Skoula, C. Abidi, E. Kokkalou, “Essential oil variation of Lavandula stoechas L. ssp. stoechas growing wild in Crete (Greece),” Biochem Syst Ecol., Vol. 24, pp. 255-260, Apr. 1996.
[22] K.A. Hammer, C.F. Carson, T.V. Riley, “Antimicrobial activity of essential oils and other plant extracts,” J Appl Microbiol., Vol. 86, pp. 985-990. Dec. 1999.
[23] L. Hui, L. Huan, L. XiaoLan, Z. AiGuo, “Chemical composition of lavender essential oil and its antioxidant activity and inhibition against rhinitis-related bacteria,” Afr J Microbiol Res., Vol. 4, pp. 309-313. Jan. 2010.
[24] L.T. Danh, L.N. Han, N.D.A. Triet, J. Zhao, R. Mammucari, N. Foster, “Comparison of Chemical Composition, Antioxidant and Antimicrobial Activity of Lavender (Lavandula angustifolia L.) Essential Oils Extracted by Supercritical CO2, Hexane and Hydrodistillation,” Food Bioprocess Tech., Vol. 6, pp. 3481-3489, Dec. 2013.
[25] B.C. Aridoǧan, H. Baydar, S. Kaya, M. Demirci, D. Özbaşar, E. Mumcu, “Antimicrobial activity and chemical composition of some essential oils,” Arch Pharm Res., Vol. 25, pp. 860-864, Dec. 2002.
[26] M. Sokovic, J. Glamočlija, P.D. Marin, D. Brkić, L.J.L.D. Van Griensven, “Antibacterial effects of the essential oils of commonly consumed medicinal herbs using an in vitro model,” Molecules, Vol. 15, pp. 7532-7546, Oct. 2010.
[27] G.B. Zore, A.D. Thakre, S. Jadhav, S.M. Karuppayil, “Terpenoids inhibit Candida albicans growth by affecting membrane integrity and arrest of cell cycle,” Phytomedicine, Vol. 18, pp. 1181-1190. Oct. 2011.