{ "article_id": 813, "public_id": "8e4438c4-0567-4ebc-8b35-2ebbb084581a", "internal_identifier": null, "source_identifier": "39:178", "status": "published", "type": "research_article", "title": "Antibiotic Resistance Properties of Chicken meat and Ground Beef Origin Pseudomonas aeruginosa", "title_secondary": null, "abstract": "Chicken and ground beef samples collected from Samsun province of Turkey were analyzed for P. aeruginosa. The\r\nisolates were obtained from classic culture technique using Pseudomonas Agar base (Oxoid CM 0559, suppl. SR 102E). After\r\nisolation, Gram staining, oxidase and catalase test and Endo Agar Base were used for the confirmation of the isolates. Catalase\r\nand oxidase positive, Gram negative and colorless colonies (up to 5) on the Endo Agar base were chosen as a presumptive P.\r\naeruginosa. Then, for the confirmation of the isolates based on molecular level, oprL gene (species-specific) was detected in the\r\nisolates using PCR assay. The oprL gene present isolates were evaluated as P. aeroginosa. In this way, 23 P.aeruginosa isolates\r\nwere obtained. Then, antibiotic resistance properties of the isolates were investigated using disk diffusion test. For this, a total 8\r\nantibiotics- ceftazidime (30 µg), colistin (10 µg), fluoroquinolones (levofloxacin) (5 µg), tobramycin (10 µg), gentamicin (10\r\nµg), meropenem (10 µg), imipenem (10 µg) and piperacillin-tazobactam (100/10 µg)- were used as antibiotics. For determination\r\nof antibiotic resistance properties of the isolates, Mueller Hinton Agar was used. According to analyzed results; 5 out of 23\r\nisolates (21.73%) were resistance to one or two antibiotics. One isolate was resistance against gentamycin, 4 (17.39%) isolates\r\nresistance to colistin. On the other hand, one of them was resistance to both gentamycin and tobramycin, too. So, 1 of 23 (4.34%)\r\nisolate resistance to aminoglycoside group antibiotics (gentamicin and tobramycin). However, 18 isolates were sensitive to all\r\ntested antibiotics. No multi antibiotic resistance isolates were present  ", "language": "en", "english_title": null, "english_abstract": null, "doi": null, "doi_url": null, "resource_url": "https://www.set-science.com/a/8e4438c4-0567-4ebc-8b35-2ebbb084581a", "pdf_url": "https://www.set-science.com/a/8e4438c4-0567-4ebc-8b35-2ebbb084581a/pdf", "og_image": "https://www.set-science.com/assets/img/og-card-v2.png", "authors": [ { "public_id": "e8514093-95cc-4e8d-ab15-5e22dc4e70a1", "given": "Belgin", "family": "Sırıken", "full": "Belgin Sırıken", "literal": null, "orcid": null, "orcid_url": null, "email": "bsiriken@omu.edu.tr", "corresponding": true, "sequence": "first", "affiliations": [ { "name": "Ondokuz Mayıs University", "department": null, "country": null, "country_code": null, "ror": null, "ror_url": null } ] }, { "public_id": "edee31b8-8966-44f9-a17a-27903f0542a0", "given": "Ceren", "family": "Başkan", "full": "Ceren Başkan", "literal": null, "orcid": null, "orcid_url": null, "email": "bsiriken@omu.edu.tr", "corresponding": false, "sequence": "additional", "affiliations": [ { "name": "Ondokuz Mayıs University", "department": null, "country": null, "country_code": null, "ror": null, "ror_url": null } ] } ], "keywords": [ "Chicken meat, ground beef, Pseudomonas aeruginosa, oprL gene, antibiotic resistance" ], "translations": [ { "language": "en", "title": "Antibiotic Resistance Properties of Chicken meat and Ground Beef Origin Pseudomonas aeruginosa", "abstract": "Chicken and ground beef samples collected from Samsun province of Turkey were analyzed for P. aeruginosa. The\r\nisolates were obtained from classic culture technique using Pseudomonas Agar base (Oxoid CM 0559, suppl. SR 102E). After\r\nisolation, Gram staining, oxidase and catalase test and Endo Agar Base were used for the confirmation of the isolates. Catalase\r\nand oxidase positive, Gram negative and colorless colonies (up to 5) on the Endo Agar base were chosen as a presumptive P.\r\naeruginosa. Then, for the confirmation of the isolates based on molecular level, oprL gene (species-specific) was detected in the\r\nisolates using PCR assay. The oprL gene present isolates were evaluated as P. aeroginosa. In this way, 23 P.aeruginosa isolates\r\nwere obtained. Then, antibiotic resistance properties of the isolates were investigated using disk diffusion test. For this, a total 8\r\nantibiotics- ceftazidime (30 µg), colistin (10 µg), fluoroquinolones (levofloxacin) (5 µg), tobramycin (10 µg), gentamicin (10\r\nµg), meropenem (10 µg), imipenem (10 µg) and piperacillin-tazobactam (100/10 µg)- were used as antibiotics. For determination\r\nof antibiotic resistance properties of the isolates, Mueller Hinton Agar was used. According to analyzed results; 5 out of 23\r\nisolates (21.73%) were resistance to one or two antibiotics. One isolate was resistance against gentamycin, 4 (17.39%) isolates\r\nresistance to colistin. On the other hand, one of them was resistance to both gentamycin and tobramycin, too. So, 1 of 23 (4.34%)\r\nisolate resistance to aminoglycoside group antibiotics (gentamicin and tobramycin). However, 18 isolates were sensitive to all\r\ntested antibiotics. No multi antibiotic resistance isolates were present  \r\n", "keywords": [ "Chicken meat, ground beef, Pseudomonas aeruginosa, oprL gene, antibiotic resistance" ] } ], "subjects": [], "subject_names": [], "published_date": "2018-12-31", "published_year": "2018", "received_date": null, "accepted_date": null, "volume": "3", "issue": null, "page_first": "929", "page_last": "932", "article_number": null, "conference": { "short_name": "ISAS2018-Winter", "full_title": "2nd International Symposium on Innovative Approaches in Scientific Studies", "name": "2nd International Symposium on Innovative Approaches in Scientific Studies", "acronym": "ISAS2018-Winter", "location": "Samsun, Türkiye", "start_date": "2018-11-30", "end_date": null, "public_id": "b8a703cc-09f7-4d5b-ae60-eb31a1050720" }, "proceedings": { "title": "2nd International Symposium on Innovative Approaches in Scientific Studies", "series_title": "Setsci conference indexing system", "publisher": "SET Technology", "isbn": null, "eisbn": null, "issn": "2618-6446", "eissn": null, "doi": null, "doi_prefix": null, "slug": "setsci-volume-3-2018-legacy-39", "public_id": "641f522e-9cf0-4c89-be17-c6037f3d1bca", "url": "https://www.set-science.com/p/setsci-volume-3-2018-legacy-39" }, "license": { "name": "CC BY 4.0", "url": "https://creativecommons.org/licenses/by/4.0/", "code": "CC-BY-4.0", "open_access": true }, "copyright": { "holder": "SET Technology", "year": "2018" }, "funding_statement": null, "funders": [], "references": [ { "order": 1, "raw": "I. Erol , “Gıda Hijyeni ve Mikrobiyolojisi”, Pozitif Matbacılık Ltd. Şti. Ankara, 2007.", "doi": null, "title": null, "authors": null, "source": null, "year": null, "volume": null, "pages": null }, { "order": 2, "raw": "B. Erdem, “ Pseudomonaslar”. Editör: Ustaçelebi Ş ve ark, Temel ve Klinik Mikrobiyoloji. Ankara: Güneş Kitabevi, 1999: 551-566.", "doi": null, "title": null, "authors": null, "source": null, "year": null, "volume": null, "pages": null }, { "order": 3, "raw": "H. Bilgehan, “Non-fermentatif gram olumsuz basiller”. Editör: Bilgehan H. Klinik Mikrobiyoloji. İzmir: Fakülteler Kitabevi Barış Yayınları, 2000: 175-197.", "doi": null, "title": null, "authors": null, "source": null, "year": null, "volume": null, "pages": null }, { "order": 4, "raw": "Costerton JW. The role of electron microscopy in the elucidation of bacterial structure and function. 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