DETERMINATION OF THIAMAZOLE IN TABLET FORMULATION BY USING REVERSED PHASE LIQUID CHROMATOGRAPHIC METHOD

Anti-thyroid drugs are relatively simple molecules known as thionamides, which contain a sulfhydryl group and a thiourea moiety within a heterocyclic structure [1]. Methimazole (1-methyl-2-mercaptoimidazole) is widely used drug in medicine for treatment of hyperthyroidism [2]. The HPLC methods are adequate methods to quantify a drug in pure form and its dosage form. Since HPLC is simple, specific, rapid, precise and accurate, this mehod may be successfully and conveniently adopted for routine quality control analysis of drugs in bulk and pharmaceutical dosage form. Analysis of basic drugs by HPLC can represent problems due to ineractions with stationary phase. YMC-Triart C18 has an extremely low level of metal impurities, much lower than conventional products. YMC-Triart C18's innovative surface modification technology results in excellent peak shapes even for the basic compounds that often exhibit tailing shapes on conventional silica and hybrid silica based ODS columns. In the present study, a simple, rapid and reproducible  reversed phase liquid chromatographic (RPLC) method was developed for the quantification of thiamazole (methimazole) in pharmaceutical dosage form. The chromatographic separation was performed using a YMC Triart C18 (150 mm X 4,6 mm, 3μm) column under the following chromatographic conditions: column temperature: 25°C, the flow rate: 0.8 mL/min. The detection wavelength of thiamazole was 240 nm and metronidazole was chosen as an internal standard. The calibration graph shows that linear response was obtained over the range of concentrations used in the assay procedure. The proposed RPLC method was successfully applied to the determination of thiamazole in commercial tablet formulation.