BİYOPOLİMER ESASLI KRİYOJEL DOKU İSKELELERİ

The new material type is ‘cryogels’, which have important potential in biotechnology applications. Cryogels can be obtained from natural or synthetic polymers. It is seen that cryogens prepared from natural polymers become a preferred material group for tissue engineering applications due to their biocompatibility and biodegradability, their toxic effects, macroporous three-dimensional structure and elastic properties that facilitate cell adhesion and cell growth [1]. In this study, the chitosan-co-hyaluronic acid cryogels at different composition were prepared by cryogelation technique. The cell viability of the cryogels were also performed for 3T3 fibroblast cells. Glutaraldehyde was used as a crosslinker for chitosan cryogels. To determine the optimum cross-linker ratio, the crosslinked chitosan cryogels were prepared with various concentrations glutaraldehyde solutions by cryogelation technique. The chitosan-co-hyaluronic acid cryogels containing hyaluronic acid at different concentrations were prepared by the same method. The prepared cryogels were investigated the properties of structural, morphological, mechanical and thermal with FTIR, SEM, DSC, TGA and compression test. The cell viability were performed for 3T3 fibroblast cells. As a result of SEM analysis, chitosan-co-hyaluronic acid cryogels were found to have high porosity due to their macropores and interconnected pore structure. The structure was given flexibility by adding hyaluronic acid into the composition. It has also been observed that the pore diameter dramatically increases. The addition of hyaluronic acid led to thinning of pore walls. According to MTT results, 3T3 fibroblast cells adhered to cryogel-based tissue scaffold and showed proliferation.

The new material type is ‘cryogels’, which have important potential in biotechnology applications. Cryogels can be obtained from natural or synthetic polymers. It is seen that cryogens prepared from natural polymers become a preferred material group for tissue engineering applications due to their biocompatibility and biodegradability, their toxic effects, macro-porous three-dimensional structure and elastic properties that facilitate cell adhesion and cell growth [1]. In this study, the chitosan-co-hyaluronic acid cryogels at different composition were prepared by cryogelation technique. The cell viability of the cryogels were also performed for 3T3 fibroblast cells. Glutaraldehyde was used as a crosslinker for chitosan cryogels. To determine the optimum cross-linker ratio, the crosslinked chitosan cryogels were prepared with various concentrations glutaraldehyde solutions by cryogelation technique. The chitosan-co-hyaluronic acid cryogels containing hyaluronic acid at different concentrations were prepared by the same method. The prepared cryogels were investigated the properties of structural, morphological, mechanical and thermal with FTIR, SEM, DSC, TGA and compression test. The cell viability were performed for 3T3 fibroblast cells. As a result of SEM analysis, chitosan-co-hyaluronic acid cryogels were found to have high porosity due to their macropores and interconnected pore structure. The structure was given flexibility by adding hyaluronic acid into the composition. It has also been observed that the pore diameter dramatically increases. The addition of hyaluronic acid led to thinning of pore walls. According to MTT results, 3T3 fibroblast cells adhered to cryogel-based tissue scaffold and showed proliferation.